Fig. 1 |. Overview of the procedure.

Experimental flow chart of described protocols. Column 1 describes steps relevant to nIRCat nanosensor synthesis. Column 2 describes steps relevant to nIRCat imaging in acute brain slices. Column 3 describes steps relevant to time series nIRCat imaging data. Dark blue boxes represent experimental steps, whereas light blue boxes represent additional experimental steps that can be added or removed depending on the planned experiment. Green boxes represent characterization steps, and red boxes represent checkpoint steps. N, no; Y, yes.