. Author manuscript; available in PMC: 2023 Sep 17.

Published in final edited form as: Nat Aging. 2023 Jul 3;3(7):776–790. doi: 10.1038/s43587-023-00446-6

Table 1 |.

Comparison of spatial proteomic methods

Platform	Supplier	Compatible tissue preparation	Max plexity	Resolution limit	Tissue area limit	Standard FOV	Stitch capability	Cyclic/all in one		Reference
Platform	Supplier	Compatible tissue preparation	Max plexity	Resolution limit	Tissue area limit	Standard FOV	Stitch capability	Stain	Detection	Reference
PhenoCycler (fmr. CODEX)	Akoya Biosciences	FFPE, FF	>100	0.25 μm	612 mm²	0.5 × 0.7 mm	Yes	All in one	Cyclic	101
CosMx SMI	NanoString	FFPE, FF	64–100	<100 nm	375 mm²	0.7×0.9 mm	Yes	All in one	Cyclic	50
MIBI-TOF	Ionpath	FFPE, FF	>40	350 nm	>800 mm²	0.8×0.8 mm	Yes	All in one	All in one	102
IMC/Hyperion Imaging System	Standard BioTools (fmr. Fluidigm)	FFPE, FF	>40	1 μm	>675 mm²	0.25×0.25 mm	Yes	All in one	All in one	103
MACSima	Miltenyi	FFPE, FF cells	>100	106 nm	>800 mm²	0.3 × 0.5 mm	Yes	Cyclic	Cyclic	104
CellScape	Canopy	FFPE, FF cells	75	182 nm	320 mm²	0.796–2.714 mm²	Yes	Cycle	Cyclic	105

FF, fresh frozen; fmr., formerly; FOV, field of view; max, maximum.