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. 2023 Aug 10;9(9):1558–1571. doi: 10.1038/s41477-023-01489-8

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — Comparison of transformation efficiencies of LAB, QLD and Northern Territory (NT) accessions. (a) Regeneration, selection, shoot development, and root development of LAB, NT and QLD ecotypes post-transformation with a 35S:Cas9 cassette and kanamycin selectable marker (scale bar represents 1 cm). The dates on top of the image indicate the progression of transformation. (b) Comparison of time taken for regeneration, growth (1-2 cm shoots) and rooting of LAB, QLD and NT. ANOVA two-tailed test was performed to determine the significance differences. (Data are presented as mean values +/− standard error (n=3 biologically independent samples). (C) Comparison of regeneration frequency and transformation efficiency of LAB, QLD and NT. ANOVA two-tailed test without transformation was performed to determine the significance differences between percentage data derived from count data. Independent positive transformants of LAB n=72, QLD n=74 and NT n=21 (a single sister plant derived from one single callus) were used to calculate the transformation efficiency.