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. 2023 Aug 10;9(9):1558–1571. doi: 10.1038/s41477-023-01489-8

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Cladogram of relationships of the nicotine demethylase genes in S. lycopersicum, N. sylvestris, N. tabacum, N. tomentosiformis, N. attenuata, and N. benthamiana (LAB and QLD). The highlighted clade contains the N. benthamiana CYP82E2 gene. Genes without stars represent proteins of uncharacterized nicotine N-demethylase activity. (B) Location of bZIP transcription factor binding motifs (red and purple triangles) in LAB and QLD 2kb promoter. The bottom panel shows the transversion in the third TF binding motif (purple triangles) that probably inhibits TF binding and expression of CYP82E2 in LAB. (C) Gene expression (TPM) of CYP82E2 in leaf and flower tissues of LAB and QLD. Error bars represent the standard error of the mean (n=3 biologically independent flower and leaf samples of LAB and QLD).