Table 1.

Genome assembly metrics of LAB and QLD compared with reference genomes

Species/accession	Scaffolds >500 nucleotides	Chromosome	L50	N50 (Mb)	Assembled genome length (Gb)	BUSCO % Complete v10
N. benthamiana LAB	19	19	10	145	2.75	C:98.1%[S:46.0%,D:52.1%]
N. benthamiana QLD	19	19	10	141	2.72	C:98.0%[S:47.5%,D50.5%]
Arabidopsis thaliana	5	5	3	23	0.12	C:99.2%[S:98.7%,D:O.5%]
Potato (dihaploid)	12	12	6	59	0.74	C:98.4%[S:96.6%,D:1.8%]
Tomato	12	12	6	61	0.72	C:97.8[S:96.8%,D:1.0%]
Eggplant	12	12	5	76	0.83	C:84.2%[S:82.7%,D1.5%]
Tobacco chromosomes (scaffolds)	24 (942,183)	24	9 (3,998)	84 (0.22)	1.74 (4.01)	C:82.6%[S:61.2%,D:21.4%] (C:96.8% [S:24.3%, D:72.5%1])
Capsicum	12	12	6.00	221	2.56	C:74.8%[S:73.7%,D:1.1%]
N. attenuata	12 (37,194)	12	498 (1,627)	66 (0.45)	0.73 (2.09)	C:48.5% [S:47.4%, D:1.1%] (C:98.1% [S:95.9%, D:2.2%])
Petunia axilaris	17,630	12	17,630	1.24	1.20	C:98.2%[S:95.6%,D:2.6%]
N. benthamiana LAB (USA vI.0.I)	52,890	19	1,718	0.44	2.49	C:98.2%[S:45.8%,D:52.4%]
Petunia inflata	35,907	12	35,907	0.88	1.17	C:97.9%[S:91.6%,D:6.3%]
N. benthamiana LAB (AU v0.5)	77,255	19	1,903	0.39	2.49	C:97.6%[S:47.5%,D:50.1%]
N. sylvestris	125,957	12	7,255	0.08	2.01	C:95.1%[S:93.3%,D:1.8%]
N. tomentosiformis	90,682	12	5,563	0.15	1.62	C:94.4%[S:92.6%,D:1.8%]
N. obtusifolia	20,758	12	2,189	0.05	3.50	C:94.3%[S:92.3%,D:2.0%]
N. otophora	420,947	12	14,141	0.03	2.32	C:76.0%D[S:74.3%,D:1.7%]

Various genome assembly quality criteria (L50, N50, BUSCO score) are used to compare N. benthamiana with the other available genomes. The values in parentheses for tobacco and N. attenuata are those obtained from scaffold data alone. L50, count of smallest number of sequences whose length sum makes up 50% of the genome assembly.