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. 2023 Aug 28;9(9):1419–1438. doi: 10.1038/s41477-023-01491-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a) Photographs of the main experimental setups n1, n2, and n3 (III) with temperature conditions ranging from 8.6 to 29.0 °C after incubation on the table for 65 h. (b) Fm measurements (maximal fluorescence) using IMAGING-PAM in various table conditions, legend on the right is a false color gradient indicating fluorescence intensity. (c) Differential interference contrast (DIC) micrographs of SAG12.97 cells grown on C-Medium (growth conditions see methods: growth conditions prior to exposure to environmental conditions); at least 10 micrographs were taken, all showing similar phenotypes of the cells. (d) Differential interference contrast (DIC) micrographs of SAG12.97 under most extreme environmental conditions (four corners: samples 1, 6, 37, and 42) as well as along an irradiance gradient at 21 °C (samples 19–24) and a temperature gradient at 130 µmol photons m^-2 s^-1 (samples 3, 9, 15, 21, 27, 33, and 39); for each well, at least 10 micrographs were taken, all showing similar phenotypes of the cells.