表 1.
qRT-PCR对差异表达的mRNA的引物序列
Primer sequences of qRT-PCR for differentially expressed mRNAs
Primer | Forward (5' to 3') | Reverse (5' to 3') |
GAPDH | TCGTGGAAGGACTCATGACC | TCCACCACCCTGTTGCTGTA |
CD133 | GCCACCGCTCTAGATACTGC | TGTTGTGATGGGCTTGTCAT |
CD44 | AGCAACCAAGAGGCAAGAAA | GTGTGGTTGAAATGGTGCTG |
Lgr5 | CTCCCAGGTCTGGTGTGTTG | GAGGTCTAGGTAGGAGGTGAAG |
MUC2 | CGAAACCACGGCCACAACGT | GACCACGGCCCCGTTAAGCA |
KRT20 | TGTCCTGCAAATTGATAATGCT | AGACGTATTCCTCTCTCACTCTCATA |
FABP2 | TGGAAGGTAGACCGGAGT | AGGTCCCCCTGAGTTCAGTT |
c-MYC | CCTGGTGCTCCATGAGGAGAC | CAGACTCTGACCTTTTGCCAGG |
CCND1 | TCTACACCGACAACTCCATCCG | TCTGGCATTTTGGAGAGGAAGTG |
ASCL2 | CGCCTACTCGTCGGACGACAG | GCCGCTCGCTCGGCTTCCG |
AXIN2 | CTCCTTATCGTGTGGGCAGT | CTTCATCCTCTCGGATCTGC |