Figure 7.

Inhibition of MMP-14 partially recovers megalin abundance and albumin uptake in AEC. (A) MLE-12 cells were inoculated with IV (MOI 1) and incubated with the specific MMP14 inhibitor NSC405020 200µM (MMP14i) or vehicle (DMSO) for 24 h Total megalin amounts were analyzed by western blot. Left: representative blot. Right: Densitometry quantification normalized to beta actin. (B) PCLS were incubated in mock (M) or influenza virus (IV) conditions for 24 h in the presence of MMP14 inhibitor NSC405020 (MMP14i) 200 µM or vehicle (DMSO) and incubated with AF488-albumin for 1 h Left: Flow cytometry data showing the MFI of AF488 in AEC. Right: % of live cells per condition. n=3. (C) Gating strategy and FMO control. *p<0.05.