Figure 5. Auto‐release of HMCES‐DPCs restricts crosslink formation to physiologically relevant situations.

• A, B
  APE1 incision of an AP site protected by the indicated HMCES^SRAP‐DPC variants at ssDNA‐dsDNA junctions (A) or within dsDNA (B). Free dU‐containing DNA was incubated alone or in the presence of UDG and HMCES^SRAP for 1 h at 37°C. Next, corresponding reverse oligonucleotides were annealed to generate an ssDNA‐dsDNA junction (A) or dsDNA (B), and reactions were incubated alone or with APE1 for the indicated amount of time at 37°C prior to separation by denaturing SDS–PAGE.

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