Figure EV5. Cell viability is not affected upon overexpression of reversal‐defective HMCES variants. Related to Fig 7 .

• A–C
  HeLa T‐REx Flp‐In cells expressing the indicated doxycycline‐inducible HMCES variants with a C‐terminal mVenus‐3xFlag‐tag were grown in the presence of 1 μg/ml doxycycline, as indicated. Expression levels were analysed by Western blotting (A). Cell viability was determined using AlamarBlue cell viability assay (B), or crystal violet staining (C).

Data information: Data in (B) represent the mean ± SD of three technical replicates normalized to the mean of untreated control (mVenus‐DOX).