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. 2023 Aug 7;42(18):e111620. doi: 10.15252/embj.2022111620

Figure EV4. ADPGK‐AS1 influences lung cancer cell proliferation and apoptosis ex vivo in normal lung PCLS.

Figure EV4

  • A
    Schematic overview and experimental design of precision‐cut lung slices (PCLS) using healthy (nontumor) lung lobes and subsequent seeding with A549‐GFP cells.
  • B
    Representative images of healthy PCLS (red, autofluorescence) with A549‐GFP (green), apoptotic cells (TUNEL+, magenta), and proliferative cells (EdU+, cyan). Scale bar = 500 μm (brightfield images), 50 μm (fluorescence images on the right).
  • C, D
    Quantification of GFP+ tumor cells (A549, n = 6), EdU+ (proliferative, n = 5), and TUNEL+ (apoptotic, n = 4) cells on healthy PCLS treated with CM (medium control, M0, M1, or M2 macrophages transfected with antisense LNA GapmeRs specific for ADPGK‐AS1) or negative control, THP1 control (EV), and ADPGK‐AS1 overexpressing (OE) macrophages. The n values represent biological replicates, mean ± SEM, one‐way ANOVA, *P ≤ 0.05, ***P ≤ 0.001, compared with M2 GapmeR‐control, §§§ P ≤ 0.001 compared with EV control.