Table 3.
Summary of cryo‐EM data collection, refinement, and validation statistics.
| EM data collection and processing | |
| Microscope | Titan Krios |
| Camera | K3 |
| Voltage (kV) | 300 |
| Magnification | ×105,000 |
| Frames (no.) | 35 |
| Total electron dose (e−/Å2) | 51 |
| Calibrated pixel size (Å) | 0.832 |
| Defocus range (μm) | 1–2.2 |
| Movies | 3,845 |
| Initial picks (no.) | 3,271,302 |
| Refined particles (no.) | 31,420 |
| Symmetry imposed | C1 |
| Global resolution (Å) | |
| FSC 0.5 (unmasked/masked) | 7.0/3.2 |
| FSC 0.143 (unmasked/masked) | 3.8/2.9 |
| Local resolution range (Å) | 2.7–5.2 |
| Map sharpening B factor (Å2) | −80 |
| EMD accession number | EMD‐40522 |
| Model refinement and validation | |
| Initial models | 3LZ0 (nucleosome); Vasudevan et al, 2010 |
| 8SIU (ORCAWD40·Orc2N) | |
| Model composition | |
| Non‐hydrogen atoms | 14,844 |
| Protein residues | 1,118 |
| DNA residues | 294 |
| Root mean square deviation | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 0.854 |
| B factors (Å2) | |
| Protein | 62.78 |
| DNA | 55.46 |
| Ramachandran plot | |
| % favored | 97.24 |
| % allowed | 2.76 |
| % outliers | 0.0 |
| Rotamer outliers (%) | 1.38 |
| MolProbity | |
| Clashscore | 5.69 |
| MolProbity score | 1.56 |
| Model‐map comparison | |
| EM Ringer score | 3.65 |
| CCmask | 0.83 |
| FSCmodel/map 0.5 | 3.1 |
| PDB accession number | 8SIY |