Figure 1.

Effects of paclitaxel (PTX) and RNA aptamer Apt 14 on porcine aortic smooth muscle cell (SMC) migration

Cells were cultured to confluence followed by two days of serum starvation, subjected to scratch wound injury, and treated with fetal bovine serum (FBS; 5%) (A) or platelet-derived growth factor-BB (PDGF; 5 ng/mL) (C) following PTX (1–1,000 nM) or with Apt 14 (200 nM) following PDGF (5 ng/mL) (E). Migration was assessed on the basis of wound confluence using the IncuCyte S3 system. (B, D, and F) Representative cell-phase images show wound confluence at 0 and 22 h after FBS or PDGF exposure. The initial scratch wound and scratch wound areas are masked in blue and yellow, respectively. Migration data were analyzed using two-way ANOVA and repeated measures with Bonferroni post-tests. ∗p < 0.05 and ∗∗p < 0.01 vs. FBS or PDGF; ^#p < 0.05 and ^##p < 0.01 vs. vehicle.