Figure 2.

Effect of paclitaxel (PTX) on porcine aortic endothelial cell migration

Cells were deprived of serum for two days and then subjected to scratch wound injury and treatment. Migration was assessed on the basis of wound confluence using the IncuCyte S3 system. Cells were treated with fetal bovine serum (FBS; 2%) (A) or platelet-derived growth factor-BB (PDGF; 5 ng/mL) (C) following PTX (1–1,000 nM) or PTX alone (E). (B, D, and F) Representative cell-phase images showing wound confluence at 0 and 22 h after treatment. The initial scratch wound and scratch wound areas are masked in blue and yellow, respectively. Data were analyzed using two-way ANOVA and repeated measures with Bonferroni post-tests. ∗p < 0.05 and ∗∗p < 0.01 vs. FBS or PDGF; ^#p < 0.05 and ^##p < 0.01 vs. vehicle.