Figure 3.

Effect of Apt 14 on porcine aortic endothelial cell migration

Cells were serum starved for 2 days, subjected to scratch wound injury, and treated with Apt 14 (200 nM) alone (A) or with Apt 14 followed by platelet-derived growth factor-BB (PDGF; 5 ng/mL) (C). The wound confluence was calculated using the IncuCyte S3 system. (B and D) Representative cell-phase images showing wound confluence at 0 and 22 h after treatment. The initial scratch wound and scratch wound areas are masked in blue and yellow, respectively. Migration data were analyzed using two-way ANOVA and repeated measures, with Bonferroni post hoc tests.