Figure 4.

Effects of paclitaxel (PTX) and Apt 14 on porcine aortic endothelial cell proliferation

Cells were treated for 3 days with fetal bovine serum (FBS; 2%) (A) or platelet-derived growth factor-BB (PDGF; 10 ng/mL) (B) following PTX (1–1,000 nM) or Apt 14 (200 nM) (C). Propidium iodide (PI) nucleic acid stain-labeled nuclei were used for cell counting. (D) Representative images of PI-positive nuclei 3 days after treatment. Data were analyzed using one-way ANOVA with Tukey’s post hoc test. ∗∗p < 0.01 vs. FBS or PDGF; ^#p < 0.05 and ^##p < 0.01 vs. vehicle. Scale bar: 100 μm.