Figure 3.
ChREBP deficiency inhibits lipogenesis and alleviates mitochondrial fission in podocytes in diabetic mice.A, representative micrographs showing glomerular expression of synaptopodin and adipophilin from 20-week-old mice. Scale bar represents 50 μm in row 1; scale bar represents 10 μm in row 2. B, representative TEM images showing mitochondrial morphology in podocytes from dbm/ChREBPPod-f/f and db/db/ChREBPPod-f/f mice obtained from 20-week-old mice. Scale bars represent 0.5 μm. The lowest panel shows tracings of mitochondria in the middle panel. C–F, mitochondrial morphology was quantitated from TEM micrographs as the (C) mitochondrial length, (D) mitochondrial aspect ratio, (E) mitochondrial form factor, (F) mitochondrial circularity. Results are presented as mean ± SEMs. ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ChREBP, carbohydrate-response element-binding protein; NS, not significant; TEM, transmission electron microscopy.