Tazemetostat-mediated H3K27me3 reduction and activation of bivalent domains are associated with cAMP, WNT, and Hedgehog pathway. Heatmap representation of number of genes with H3K27me3 (A) or H3K4me3 (B) enrichment in different distances away from TSS. Graphical representation of the fold change (EPZ6438 versus Veh) of H3K27me3 (C) and H3K4me3 (D) enrichment in relationship with genomic distribution. Gene ontology of genes with H3K27me3 reduction (E) and H3K4me3 (F) enrichment using DAVID. Schematic representation of cAMP, WNT, and Hedgehog associated with pathways in cancer (G). Red outline indicates genes with H3K27me3 reduction and H3K4me3 enrichment. TSS, transcriptional start site.