Skip to main content
. 2023 Aug 11;299(9):105155. doi: 10.1016/j.jbc.2023.105155

Figure 7.

Figure 7

Silencing of Gli1 and Gli2 increases the mineralization inMC3T3preosteoblast pretreated with EPZ6438. Diagram of the experimental protocol for treatment of MC3T3 with siCtrl, siGli1, or siGli2 and with vehicle (Veh) or 2.5 μM EPZ6438 shown in panels B–D (A). Gli1 and Gli2 mRNA levels as evaluated by RT-qPCR on day 5 (B). RT-qPCR analysis for bone-related markers relative to Gapdh (set at 100) on day 7 (C). Alizarin red staining on day 10 (D). All error bars represent ±SD of three experimental replicates (ns = not significant, ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, and ∗∗∗∗p ≤ 0.0001). RT-qPCR, real-time quantitative PCR.