Figure 3.

TCR of endogenous NNAT mRNA.A, Western blot of lysates from Neuro-2a cells transfected with control shRNA or NNAT-targeting shRNAs performed using anti-NNATx antibody. Densitometry graph shows relative density (mean ± SD, p value by paired two-tailed Student’s t test) from three experiments. RT-PCR result showing NNAT mRNA levels in these cells is shown below. B, Western blot of mouse pup brain lysate showing the expression of NNATx in vivo. C, NNAT ribosome profile in mouse embryonic neural stem cells (SRR1382093) showing the presence of ribosomes in the ISR. Position of the start codon (green) and the two in-frame stop codons (red) are shown. Inset shows density of reads in the ISR and the 3′ UTR of mouse NNAT (N = 15 datasets). Statistical significance was calculated using two-tailed Student’s t test with Welch’s correction. TCR, termination codon readthrough.