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. 2023 Aug 22;299(9):105184. doi: 10.1016/j.jbc.2023.105184

Figure 6.

Figure 6

NNATx does not induce neuronal differentiation.A and B, qRT-PCR results showing the expression of CHAT (A) and RBFOX3 (B) mRNAs relative to ACTB mRNA in Neuro-2a cells overexpressing NNAT or NNATx. The graphs (mean ± SD) are representatives of three independent experiments performed with triplicate samples. Statistical significance was calculated using two-tailed Student’s t test. C, quantification of neurites length (mean ± SD) in Neuro-2a cells overexpressing NNAT or NNATx. At least 50 cells in each group from four biological replicates were analyzed to quantify the neurites. Representative images are shown. Cells were treated with differentiation medium containing 2 μM retinoic acid for 2 days. NS, not significant; CHAT, choline acetyltransferase.