Table 2.

Sequencing statistics across all samples

Metric	Parameter	Mean ± SD
Post-QC sequencinga	Reads per sample (thousands)	713 ± 215
	Average read length (bp)	80.8 ± 1.5
	Median read length	91.6 ± 0.5
	Percent of reads ≥ 65 bp	79.3 ± 3.3%
Reference-based	Assignment rateb	20.8 ± 3.5%
Reference-free	Number of tagsc (thousands)	186
	Assignment ratec	29.2 ± 3.8%

^aAfter demultiplexing and trimming; QC: Phred quality score of at least 20 and read length of at least 40 bp

^bPercent of reads that were assigned to the reference database, consisting of the Hungate1000 Collection plus four Quinella genomes

^cPercent of reads that were assigned to a tag, i.e., unique 65 bp reads, starting from the cut site, that are present in at least 25% of all samples