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. 2023 Sep 18;23:879. doi: 10.1186/s12885-023-11358-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Four EMT-related genes affect EC cell metastasis. A-B Validation of silencing efficiency of four EMT-related genes in HEC-1-B and Ishikawa cell lines. C Migration detected by wound healing assays in HEC-1-B cell line. D Migration detected by wound healing assays in Ishikawa cell line. E Migration detected by transwell migration assay in HEC-1-B and Ishikawa cell lines. F Invasion detected by transwell invasion assay in HEC-1-B and Ishikawa cell lines. The means of two independent samples were compared for a statistically significant difference by the unpaired t-test. (*P < 0.05, **P < 0.01, ***P < 0.001, ns: no significant)