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. Author manuscript; available in PMC: 2023 Nov 30.
Published in final edited form as: Sci Transl Med. 2023 May 31;15(698):eabo3189. doi: 10.1126/scitranslmed.abo3189

Fig. 2: Pan-neuronal RNAi-mediated knockdown of PSMC3/Rpt5 results in normal learning performance but defective reversal learning performance.

Fig. 2:

A. Shown is an illustration of a T-maze used for conditioning of odor-avoidance in Drosophila. Flies were trained to avoid one particular odor chamber that was associated with a foot-shock (in this example, odor chamber 1). B. The time course of the learning and reversal learning protocols used in these experiments is illustrated. Reversal learning was assessed by reversing odor shock pairing (4-methylcyclohexanol, MCH−; 3-octanol, OCT+), as indicated. C. Upper left: Shown is the learning performance index of wildtype flies (WT), flies expressing elav, flies with full expression of Rpt532422 RNAi (WT: Rpt532422) and flies with pan-neuronal expression of Rpt532422 RNAi (Elav: Rpt532422) (P=0.6435, N=4). Upper right: Shown is the learning performance index of wildtype flies (WT), flies exressing elav (WT:Elav), flies with full expression of Rpt553886 RNAi (WT: Rpt553886) and flies with pan-neuronal expression of Rpt553886 RNAi (Elav: Rpt553886) (P=0.5282, N=6; Upper right). Lower left: Shown is the reversal learning performance of all group described in C, Upper left. (P<0.0001, N=4). Lower right: Shown is the reversal learning performance index for all groups descripted in C, Upper right (P=0.0022, N=6). Statistical analysis was performed using ANOVA and then Tukey tests in JMP (SAS).**, P<0.01; ***, P<0.001.