Fig 10.

Major activated canonical pathways in eight areas of the brain from mice intranasally (i.n.) infected with VEEV TC-83 at 1, 3, 5, 6, and 7 days post-infection (dpi). Mice (n = 4) were i.n. infected with VEEV TC-83 or mock inoculate with PBS. At 1, 3, 5, 6, and 7 dpi brains were harvested from VEEV TC-83-infected mice and at 1 and 5 dpi brains were harvested from mock-infected mice for RNA-Seq. Differential gene expression was calculated using DESeq2 for each day of the VEEV TC-83-infected samples using all of the mock-infected mice as controls. Differentially expressed genes (Log₂ fold change ≤−2 and ≥2; false discovery rate ≤0.05) were used to assess canonical pathways activation using Ingenuity Pathway Analyses. The activation score is noted in the color bar below the figure. Abbreviations: CBX, cerebellum; HIP, hippocampus; MOB, main olfactory bulb; MTX, motor cortex; PIR, piriform cortex; STR, striatum; STX, sensory cortex; THA, thalamus.