Fig 3.

Representative immunohistochemical images of seven areas of the mouse brain probed for the presence of VEEV TC-83 glycoprotein (GP) from 1- to 7 days post-infection (dpi) and mock inoculated. Five- to six-week-old, female, C3H/HeN mice were inoculated intranasally with PBS (n = 4/day) or 10⁷ PFU of VEEV TC-83 (n = 4/day) and were sacrificed humanely on 1, 2, 3, 4, 5, 6, and 7 dpi. The mock-inoculated tissues are shown in the first row of the figure for each section of the brain. Each area of the brain is labeled at the top of the figure and the day post-infection is shown to the left of the figure. Seven regions of the brain are presented in the images: the MOB—main olfactory bulb; PIR—piriform cortex; STR—striatum; MTX—motor cortex; HIP—hippocampus; THA—thalamus; CBX—cerebellum. Images are at 20× magnification. VEEV GP was detected by immunohistochemistry of brains from mice, which is indicated by a dark brown color in the images. Images were chosen from sagittal sections that were cut with a Leica CM3050 at 40 µM thickness. Scale bar = 50 µm.