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. Author manuscript; available in PMC: 2023 Sep 18.
Published in final edited form as: J Leukoc Biol. 2021 Jul 16;111(4):805–816. doi: 10.1002/JLB.2A1220-824R

FIGURE 3. miR-181c expression results in decreased myeloid cell leukemia (MCL)1 level and increased cell death.

FIGURE 3

GFP-tagged miR-181c expression plasmid was transfected into HL-60 cells. GFP-positive cells were sorted and collected by FACS sorter 48 h after transfection. Increased miR-181c level (A) and decreased MCL1 mRNA level (B) were observed by quantitative real-time PCR (qRT-PCR) in GFP-positive HL-60 cells 48 h after transfection. Aliquots of same volume of transfected cells were counted for live GFP-positive HL-60 cells (C). Mean and SD of triplicate experiments are shown. **, P < 0.01