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. Author manuscript; available in PMC: 2023 Sep 18.

Published in final edited form as: Bioconjug Chem. 2022 Apr 7;33(4):718–725. doi: 10.1021/acs.bioconjchem.2c00083

Table 1.

Key Spectroscopic Properties

CyMask-CO₂H	λ_max,abs (nm)	λ_max,em (nm)	Φ_F	ε^a (M⁻¹ cm⁻¹)	brightness (ε × Φ_F)	net charge	molecular weight
CyMask-Me-CO₂H (6a)	766	781	0.14	161,000	22,500	0	779.0
CyMask-PEG-8-CO₂H (6b)	766	781	0.13	203,000	26,400	0	1483.9
CyMask-PEG-24-COOH (6c)	766	781	0.15	182,300	27,500	0	2893.6
CyMask-Sulfo-CO₂H (6d)	764	780	0.11	174,000	19,000	-2	965.4
CyMask-Quat-CO₂H (6e)	764	780	0.12	184,000	22,000	+2	923.3
CyMask-Zwit-CO₂H (6f)	764	780	0.11	184,000	20,240	0	1165.5

^a

Molar absorption coefficients of all dyes were measured in 1:1 MeOH-PBS pH 7.4.