. Author manuscript; available in PMC: 2023 Sep 18.

Published in final edited form as: Biomed Pharmacother. 2022 Apr 5;149:112922. doi: 10.1016/j.biopha.2022.112922

Table 1.

Cytotoxicity of SKLB610 in human cell lines overexpressing ABCB1 or ABCG2.

Cell line	Type	Transporter overexpressed	IC₅₀ (μM)^a	RF^b

KB-3–1	Epidermal	–	15.75 ± 2.48	1.0
KB-V1	Epidermal	ABCB1	10.75 ± 2.32	0.7
OVCAR-8	Ovarian	–	20.92 ± 1.31	1.0
NCI-ADR-RES	Ovarian	ABCB1	25.07 ± 2.64	1.2
H460	Lung	–	31.07 ± 3.22	1.0
H460-MX20	Lung	ABCG2	25.10 ± 2.76	0.8
S1	Colon	–	16.06 ± 4.15	1.0
S1-MI-80	Colon	ABCG2	17.06 ± 2.26	1.1
pcDNA3.1-HEK293	–	–	54.72 ± 7.46	1.0
MDR19-HEK293	–	ABCB1	68.61 ± 12.58	1.3
R482-HEK293	–	ABCG2	77.76 ± 12.89	1.4

Abbreviations: RF, resistance factor.

IC₅₀ values were calculated from dose-response curves obtained from at least three independent experiments as described in Section 2.

RF values were obtained by dividing the IC₅₀ value of SKLB610 in the ABCB1- or ABCG2-overexpressing multidrug-resistant cell lines by the IC₅₀ value of SKLB610 in the corresponding drug-sensitive parental cell lines.