. Author manuscript; available in PMC: 2024 Jun 19.

Published in final edited form as: Chem Res Toxicol. 2023 May 15;36(6):870–881. doi: 10.1021/acs.chemrestox.3c00003

Table 4.

Impact of PPB and Hepatic Clearance on PFAS C_ss Estimation

PFAS Binding	f_up	f_ub	Cl_{in vitro} (μl/min*10⁶ cells)	Cl_hepatic (L/hr)	C_ss (μM)	f_up Fold-Difference	C_ss Fold-Difference
Minimum f_up	0.0001	0.00018	0	0.0000	5710.51	1	1
			3	0.0057	1000.23	1	6
			10	0.0191	342.05	1	17

25^th percentile f_up	0.0018	0.00327	0	0.0000	317.25	18	18
			3	0.1033	55.62	18	103
			10	0.3434	19.07	18	299

Median f_up	0.005	0.00909	0	0.0000	114.21	50	50
			3	0.2864	20.06	50	285
			10	0.9475	6.91	50	827

75^th percentile f_up	0.04	0.07273	0	0.0000	14.28	400	400
			3	2.2410	2.55	400	2237
			10	7.0599	0.92	400	6187

C_ss values were predicted using a generic TK model described previously (Wetmore et al., 2015), using 1mg/kg/day dose rate and TK inputs above. PFAS f_up distributions calculated for test set. Blood:plasma ratio used to calculate f_u in blood (f_ub); glomerular filtration rate (GFR) of 6.71 L/hr used to estimate renal clearance (GFR*f_ub).