Fig 6.

A3B relocalization requires de novo translation of HCMV proteins but not viral DNA synthesis. (A) Schematic representation of experimental workflows of CHX and PAA treatment of infected cells. Image created with BioRender. (B) Representative IF microscopy images of HFF-1 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with AD169-GFP and treated with DMSO or CHX for 24 h (10 µm scale). (C) Representative IF microscopy images of U373 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with AD169-GFP or AD169-GFP ΔIE1 for 72 h (10 µm scale). (D) Representative IF microscopy images of HFF-1 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with AD169-GFP and treated with DMSO or PAA for 48 h (10 µm scale). (E) Quantification of A3B-HA subcellular localization phenotypes shown in panels B and D. Each histogram bar reports the percentage of cells with cytoplasmic A3B-HA (n > 80 cells per condition; mean ± SD with P values from unpaired Student’s t-tests).