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. 2023 Aug 11;97(8):e00181-23. doi: 10.1128/jvi.00181-23

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Copyright © 2023 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 11 — FMDV 3C^pro protein principally alleviates the anti-FMDV effect of Vps28. (A and B) PK-15 cells were co-transfected with Flag-EV and HA-EV or Flag-EV and HA-Vps28 or FLAG-2B and HA-Vps28 or Flag-3A and HA-Vps28, or Flag-3C and HA-Vps28 and then infected the cells with FMDV (MOI = 1). Cells were collected and then western blot and TCID₅₀ assay were performed to detect protein expression and viral titer after FMDV infection. (C and D) PK-15 cells were transfected with Flag-EV and HA-EV or Flag-EV and HA-Vps28 or Flag-2B and HA-Vps28 or Flag-3A and HA-Vps28, or Flag-3C and HA-Vps28 and then infected the cells with FMDV (MOI = 1), 6 hours after FMDV infection, transcription levels of beta interferon (IFN-β) and IL-6 were detected by RT-qPCR. Data are means and SD of the results of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant.