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. 2023 Aug 9;97(8):e00851-23. doi: 10.1128/jvi.00851-23

Fig 1.

Fig 1

Omicron BA.1 uses TMPRSS2 less efficiently regardless of pseudovirus spike cleavage status. (A) Graphical depiction of VSV pseudovirus production and subsequent infectivity assays (made with Biorender). (B) Immunoblotting of concentrated 614G, Alpha, Delta, and Omicron BA.1 pseudovirus stocks. (C) Quantification of blots in (B). (D) Infectivity of pseudoviruses in VeroE6 and VeroE6-TMPRSS2 cells. (E) Immunoblotting the S2 of Delta and Omicron BA.1 pseudovirus grown in the presence or absence of 10% FBS and concentrated by high-speed centrifugation. (F) Infectivity of pseudoviruses produced in (E) in VeroE6 and VeroE6-TMPRSS2 cells. Groups were compared by two-way ANOVA. *P < 0.05. Error bars depict SEM. Panels D and F indicate three technical replicates. Panel C indicates quantification from three independent pseudovirus productions.