Fig 2.

Omicron BA.1 partially utilizes the cathepsin-mediated entry pathway in VeroE6-TMPRSS2 cells, and all variants enter VeroE6 cells using the cathepsin-mediated pathway. (A) Graphical depiction of VSV pseudovirus production and subsequent entry route assays (made with Biorender). (B and C) Percentage entry of pseudoviruses in VeroE6-TMPRSS2 cells pretreated with a concentration range of either E64D (B) or camostat (C). (D and E) Percentage entry of pseudoviruses in VeroE6 cells pretreated with a concentration range of either E64D (D) or camostat (E). Groups were compared by two-way ANOVA. *P < 0.05. Error bars depict SEM. Panels B–E indicate three technical replicates.