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. 2023 Aug 9;97(8):e00851-23. doi: 10.1128/jvi.00851-23

Fig 5.

Fig 5

Omicron BA.1 grown on Calu-3, VeroE6, or VeroE6-TMPRSS2 cells is equally cleaved and benefits less from TMPRSS2. (A) Immunoblotting the S1 of Delta, Omicron BA.1, and Omicron XBB.1.5 viruses grown on Calu-3 cells. (B) Viral titers on VeroE6 and VeroE6-TMPRSS2 cells of stocks produced on Calu-3 cells. (C) Immunoblotting of Delta and Omicron BA.1 stocks passaged once on VeroE6 or VeroE6-TMPRSS2 cells. (D and E) Viral titers on VeroE6 and VeroE6-TMPRSS2 cells of stocks produced on either VeroE6 (D) or VeroE6-TMPRSS2 (E) cells. Groups were compared using a two-way ANOVA. *P < 0.05. Error bars depict SEM. NP = nucleocapsid protein. Panels B, D–E indicate three technical replicates. Panel B is a representative from two independent experiments.