Fig 2.

Rta is responsible for the induction of IQGAP2 expression. (A and B) EBV-negative (Akata⁻) and EBV-positive (Akata⁺) cells were treated without or with 0.5% goat anti-human IgG. (A) RNA and (B) protein were extracted for RT-PCR and western blotting, respectively. Expression of IQGAP2, EBNA1, Zta, and β-actin was detected. (C) Expression of IQGAPs was analyzed in TW01 cells transfected with the indicated EBV viral genes. mRNA expression was assessed by RT-PCR analysis 72 h post-transfection. (D) TW01 cells were transfected with pSG5 or pSG5-Rta using NTR II, and cell lysates were harvested 72 h post-transfection. Transient expression of Rta was achieved by electroporation in BJAB cells, and cell lysates were prepared 48 h later. The obtained proteins were analyzed by western blotting to detect the expression of IQGAP2 and Rta. β-actin served as the internal control.