Fig 6.

Knockdown of IQGAP2 alters LCL clumping morphology and decreases E-cadherin expression. (A–D) LCLs were infected with shLuc-, shIQGAP2-2-, or shIQGAP2-3-expressing lentivirus for 5 d and then underwent 48 h of puromycin selection. (A) Cells were then seeded in 96-well plates and incubated for 24 h. Images of LCL clumping morphology were taken under a bright-field microscope. LCLs from (B) donor 1 and (C) donor 2 were seeded in 96-well plates, and cell viability was measured with the alamarBlue assay. Cell proliferation rate was obtained by comparing the number of viable cells over time. n.s. stands for not significant. (D) Cells were then harvested and analyzed by western blotting to detect E-cadherin expression. Anti-IQGAP2 antibody was used to confirm knockdown efficacy. β-actin served as the internal control.