Fig 2.
Stabilizing UL136p33 modestly alters viral gene expression in a productive infection. (A) MRC-5 lung fibroblast cells were infected with UL136 myc, UL136mycΔK→R, ΔUL135STOP/UL136myc, or ΔUL135STOP/UL136mycΔK→R recombinant viruses at MOI of 1. Lysates were collected over a time course of infection and immunoblotted using antibodies specific to the myc epitope tag (UL136 isoforms), UL135 (UL135 isoforms), UL44, IE1&2 (clone 3H4), pp28, and pp150. Tubulin was used as a loading control. Representative blots are shown. (B and C) Protein levels quantified over multiple independent experiments using Image Studio Lite quantification software. All protein bands are normalized to tubulin. Bars represent the averages of at least three independent experiments with standard deviation shown. Significance is calculated using a two-way ANOVA with Tukey’s multiple comparison tests. *P < 0.01; **P < 0.001; ***P < 0.0001.