Fig 4.
Increased UL136p33 concentration does not enhance viral gene expression separate from viral DNA synthesis. (A) MRC-5 cells were infected with WT UL136 myc or UL136mycΔK→R, at an MOI of 1 and treated with PAA (50 µg/mL) or ethanol as the vehicle control at the time of infection. The drug was refreshed at 24 and 48 hpi to account for decay. Lysates were collected and immunoblotted at the indicated time points for viral proteins using antibodies described in Table 1. The tubulin antibody was used as a loading control. Representative blots are shown. (B) Multiple independent experiments were quantified. Data points represent the averages from three independent experiments, and error bars represent standard deviations. A two-way ANOVA with Tukey’s multiple comparison tests were performed to determine the statistical significance values for each infection relative to PAA treatment at each time point. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.