Fig 5.
Stabilizing UL136p33 does not direct the middle isoforms of UL136 for proteasomal degradation. MRC-5 fibroblasts were infected with either UL136 myc or UL136mycΔK→R. 6 h prior to lysate collection, infected cells were treated with 20 µM MG132 to inhibit the proteasome or vehicle control. Lysates were collected at 24 hpi and immunoblotted for myc to detect protein isoforms and tubulin (as a loading control) with antibodies described in Table 2. Three independent biological replicates were used to calculate statistical significance. Statistical significance was calculated through a two-way ANOVA with Tukey’s multiple comparison tests for each isoform as follows. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.