TABLE 1.
Site-directed mutagenesis primers used in this study a
LACV strain | Primer name | Sequence | ||
---|---|---|---|---|
78/NC-cl | LACV Gc H1192A For | GATTTGACTACCTATGCgctTTAGCTAGCAGAAAG | ||
78/NC-cl | LACV Gc H1192A Rev | CTTTCTGCTAGCTAAagcGCATAGGTAGTCAAATC | ||
78/NC-cl | LACV Gc A1195V For | CTACCTATGCCATTTAgtcAGCAGAAAGGAAGTC | ||
78/NC-cl | LACV Gc A1195V Rev | GACTTCCTTTCTGCTgacTAAATGGCATAGGTAG | ||
78/NC-cl | LACV Gc R1197E For | GCCATTTAGCTAGCgagAAGGAAGTCATTG | ||
78/NC-cl | LACV Gc R1197E Rev | CAATGACTTCCTTctcGCTAGCTAAATGGC | ||
78/NC-cl | LACV EcoRI For | CAAATATGATGAATTCTGGGATG | ||
78/NC-cl | LACV NotI Rev | GCTACGCGGCCGCCTTAGCC |
Lowercase = mutant codon.