Fig 5.

Nucleoside analogs nelarabine and fludarabine reduce HIV transcripts. MDM were infected with YU-2/VSVg and efavirenz added 5 d later to prevent new rounds of infection. Four days later, they were treated with or without nelarabine (3 µM) or fludarabine (3 μM) . RNA was isolated 7 d later and assayed by RT-qPCR with primers targeting HIV tat, gag, nef and two different regions of the 5′ nascent transcript (TAR-short and TAR-long). Transcription is shown as fold over cellular GUSB levels, calculated by the ΔΔCt method.