Fig 6.

Nelarabine reduces H3K9me3 on the HIV proviral genome and cellular subtelomeres. MDM were infected with YU-2/VSVg. Seven days later, efavirenz was added to block further rounds of reinfection, and 3 d later treated with or without nelarabine (3 µM). Six days later, crosslinking was carried out and nuclei isolated. Cells were assayed by ChIP for association of H3K9me3, total histone H3, or IgG control using primers positioned throughout the HIV genome, or at control loci in the human genome (actin, GAPDH, subtelomere 10q_CTCF, and 10q_TERRA). (A) HIV-1 genome regions targeted by PCR primers; (B) H3K9me3; (C) Total histone H3; and (D) IgG control. Data are presented as percent of input DNA for each primer pair and presented as means ± SEM for n = 3 technical replicates.