GPR41 and/or GPR43 play an important role in cognition and pathology. a, Recognition index of NOR test of 5 month Gpr41/43/109a+/+, Gpr41/43/109a−/−, Gpr41/43−/−, Gpr109a−/− WT/5xFAD mice (WT Gpr41/43/109a+/+
n = 8, WT Gpr41/43/109a−/−
n = 16, WT Gpr41/43−/−
n = 7, WT Gpr109a−/−
n = 8, AD Gpr41/43/109a+/+
n = 10, AD Gpr41/43/109a−/−
n = 23, AD Gpr41/43−/−
n = 9, AD Gpr109a−/−
n = 19). b, Representative immunofluorescence images of Iba1+ microglia (magenta) and 6E10+ plaques (cyan) from the cortex of 6 month Gpr41/43/109a+/+, Gpr41/43/109a−/−, Gpr41/43−/− mice, Gpr109a−/− 5xFAD mice. Scale bar, 100 μm c, d, Average plaque number (c) and plaque size (d) in DG and cortex. AD Gpr41/43/109a+/+
n = 9, AD Gpr41/43/109a−/−
n = 10, AD Gpr41/43−/−
n = 9, AD Gpr109a−/−
n = 11. e, f, Representative immunofluorescence images (e) and quantification (f) of DCX+ newly born neurons (green) from the DG of 6 month Gpr41/43/109a+/+, Gpr41/43/109a−/−, Gpr41/43−/− mice, Gpr109a−/− 5xFAD mice. Scale bar, 100 μm. WT Gpr41/43/109a+/+
n = 10, WT Gpr41/43/109a−/−
n = 11, WT Gpr41/43−/−
n = 6, WT Gpr109a−/−
n = 10, AD Gpr41/43/109a+/+
n = 11, AD Gpr41/43/109a−/−
n = 12, AD Gpr41/43−/−
n = 10, AD Gpr109a−/−
n = 11. Data are mean ± SEM. *p < 0.05, **p < 0.01; two-way ANOVA and Tukey’s multiple comparison test. Extended Data Figure 1-1 supports Figure 1.