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. 2023 May 9;18(1):20230678. doi: 10.1515/med-2023-0678

Figure 3.

Figure 3

circ_0005615 directly interacts with miR-665. (a) Putative binding site of miR-665 in circ_0005615 is shown. (b) Overexpression efficiency of miR-665 was detected using qRT-PCR (n = 3). (c and d) Luciferase activity was tested in LOVO and SW480 cells co-transfected with WT-circ_0005615 or MUT-circ_0005615 and miRNA NC or miR-665 mimic (n = 3). (e) miR-665 level was determined in CRC tissues and adjacent normal tissues (n = 30). (f) miR-665 level was examined in NCM460, LOVO, and SW480 cells (n = 3). (g) Transfection efficiency of miR-665 inhibitor was detected by qRT-PCR (n = 3). (h) miR-665 level was measured in LOVO and SW480 cells transduced with si-NC, si-circ_0005615, si-circ_0005615 + inhibitor NC, or si-circ_0005615 + miR-665 inhibitor (n = 3). (i) Expression of miR-665 was detected in LOVO and SW480 cells treated with 6 Gy irradiation (n = 3). Data are shown as mean ± SD. *P < 0.05.