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. 2023 May 9;18(1):20230678. doi: 10.1515/med-2023-0678

Figure 5.

Figure 5

NOTCH1 is a target of miR-665. (a) Putative binding site of miR-665 in NOTCH1 3′-UTR was displayed. (b and c) Luciferase activity was detected in LOVO and SW480 cells co-transfected with WT-NOTCH1-3′-UTR or MUT-NOTCH1-3′-UTR and miRNA NC or miR-665 mimic (n = 3). (d) NOTCH1 mRNA level was examined in CRC tissues and adjacent normal tissues (n = 30). (e) NOTCH1 protein level was measured in NCM460, LOVO, and SW480 cells (n = 3). (f) NOTCH1 protein expression was determined in LOVO and SW480 cells transfected with pc-NC or pc-NOTCH1 (n = 3). (g) NOTCH1 protein level was detected in LOVO and SW480 cells transfected with miRNA NC, miR-665 mimic, miR-665 mimic + pc-NC, or miR-665 mimic + pc-NOTCH1 (n = 3). (h) NOTCH1 protein level was measured in LOVO and SW480 cells transfected with si-NC, si-circ_0005615, si-circ_0005615 + inhibitor NC, or si-circ_0005615 + miR-665 inhibitor (n = 3). (i) NOTCH1 protein level was detected in LOVO and SW480 cells treated with 6 Gy irradiation (n = 3). Data are shown as mean ± SD. *P < 0.05.