Fig 1. VHFD intake and palatability are not stable during the week.
(A) Daily intake peaked every Monday, when VHFD was replaced in the feed hopper, then gradually declined until VHFD was replaced again. At week 5, VHFD was replaced on Tuesday (arrow) rather than Monday to rule out “weekend effects.” (B) Average intake per day of the week was highest on Monday. (C) Food preference tests show that mice preferred to eat freshly thawed VHFD rather than up to 7-day old VHFD. The dotted line indicates 50% chance. All values represent AVG ± SEM. #p < 0.10, **p < 0.05, *p < 0.01, d = day.
