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. 2023 May 12;30(9):706–713. doi: 10.1038/s41434-023-00404-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Retroviral transduction efficiency of T cells determined by flow cytometry. T cells from HDs were genetically modified. Transduction efficiency was analyzed on day 7 after the beginning of production. Mean ± SD of three independent experiments. B Representative dot plots for AP1903-induced killing determined by flow cytometry. C Cell death percentages of cells expressing iCasp9-G measured 24 h after 10 nM AP1903 exposure. Normalized to control cells (untreated cells). Cell death was evaluated by Annexin-V/7-AAD labeling and gating on CD3⁺/CD19⁺ cells (−Gr) or CD3⁺/CD34⁺ cells (−Ar) by flow cytometry. Mean ± SD of three independent experiments. ****P < 0.0001 with unpaired Student t test.