Skip to main content
. 2023 May 12;30(9):706–713. doi: 10.1038/s41434-023-00404-2

Fig. 3. Functional dose improvement for suicide gene induction in RapaCasp9-expressing GMTCs.

Fig. 3

A Killing curve after 24 h of exposure to rapamycin at doses ranging from 0.1 nM to 100 nM. Mean ± SD of three independent experiments. B Cell death percentages of RapaCasp9-G-expressing GMTCs 24 and 48 h after rapamycin exposure. Cells were exposed to rapamycin at a published dose (1 nM) or clinical doses equivalents (54.5 and 109 nM). Normalized to control cells (DMSO exposure). Cell death was evaluated by Annexin-V/7-AAD labeling and gating on CD3+/CD19+ cells by flow cytometry. Mean ± SD of three independent experiments. **P < 0.01; ***P < 0.001 with Two-way Anova test. C Cell death percentages of RapaCasp9-G-expressing GMTCs during 72 h after rapamycin exposure. Cells were exposed to 109 nM rapamycin and normalized to control cells (DMSO treated). Cell death was evaluated by Annexin-V/7-AAD labeling and gating on CD3+/CD19+ cells by flow cytometry. Mean ± SD of three independent experiments.