Fig. 1. Enzyme kinetics and ITC experiments of AaNGT.

a Scheme illustrating the general reaction of NGTs. b Glycosylation kinetics of AaNGT using UDP-Glc and the peptide FGNWTT. c Plots comparing the initial velocities of AaNGT in the presence of different sugar nucleotides. Additional kinetic data are given in Supplementary Table 1. All experiments were obtained in duplicate (n = 2 independent experiments). d ITC data for the binding of UDP to AaNGT. Top: raw thermogram (thermal power versus time). Bottom: binding isotherm (normalized heats versus molar ratio). The experiment was repeated at least 2 times independently with similar results, and one representative plot for each experiment is shown. e Graph depicting the K_ds for the nucleotides (see Supplementary Table 2 for all ITC data). Source data are provided as a Source Data file.